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Image Search Results
Journal: PLoS ONE
Article Title: Screening for modulators of neural network activity in 3D human iPSC-derived cortical spheroids
doi: 10.1371/journal.pone.0240991
Figure Lengend Snippet: A) Confocal image of a 3D neurosphere showing nuclei (DAPI-blue), neurons (MAP2-green), and astrocytes (GFAP-red) demonstrating the presence of a mixture of neurons and astrocytes on the spheroid surface. B) Cryosectioned 3D sphere showing nuclei (DAPI-blue), neurons (MAP2-green) and astrocytes (GFAP-red) illustrating the presence of these cells in the interior of the spheroid. Scale bars represent 50 μm. C) Quantification of calcium dye intensity over time in a 3D culture by confocal imaging and by FLIPR. RFU = relative fluorescence units. D) Heat map of peak frequency (peak count per 10min interval) in 384 well plate from 2D and 3D cultures. E) Mean peak count across wells of 2D and 3D plates. F) Mean peak amplitude across wells of 2D and 3D plates. Error bars represent standard deviation.
Article Snippet: Two-dimensional (2D) cultures and 3D spheroids of human iPSC-derived cortical neurons and
Techniques: Imaging, Fluorescence, Standard Deviation